Description
5-(3-Aminoallyl)-2'-deoxyuridine-5'-O-triphosphate (aminoallyl-dUTP) is an amine-modified nucleotide that has been used in the synthesis of fluorescently labeled UTP.
1 Incorporation of aminoallyl-dUTP into primers has been used in the generation of fluorescently labeled probes for DNA microarrays and FRET-based assays to measure the activity of reverse transcriptases.
2,3
References
1. Kuznetsova, V.E., Spitsyn, M.A., Shershov, V.E., et al.
Novel fluorescently labeled nucleotides: Synthesis, spectral properties and application in polymerase chain reaction Mendeleev Commun. 26(2),95-98(2016).
2. Xiang, C.C., Kozhich, O.A., Chen, M., et al.
Amine-modified random primers to label probes for DNA microarrays Nat. Biotechnol. 20(7),738-742(2002).
3. Krebs, J.F., and Kore, A.R.
Novel FRET-based assay to detect reverse transcriptase activity using modified dUTP analogues Bioconjug. Chem. 19(1),185-191(2008).
