Uses
Anti-Chicken IgY (IgG) (whole molecule)-FITC antibody may be used for immunofluorescence using human peripheral blood lymphocytes at a working antibody dilution of 1:320. For immunohistochemistry a working antibody dilution of 1:320 may be used. A dilution of 1:1000 was used for EP receptor staining of murine keratinocyte cell line 308 using primary antibodies generated in chicken. The antibody was also used for immune electron microscopy labeling of porcine nerve samples.
General Description
Chicken IgY is the major antibody found in chicken serum. Chickens generate a comparatively enhanced antibody response to mammalian antigenic proteins. These immunoglobulins do not bind to Fc receptors present in mammals and bacteria, and also do not interact with rheumatoid factors. Thus chicken antibodies are useful analytical tools for various research applications .
Biochem/physiol Actions
IgY plays a major role in defense mechanism against systemic infections. It also possesses the ability to initiate anaphylactic reactions.
