Uses
The combination of PCR Buffer without MgCl
2 and the MgCl
2 Stock Solution buffer are optimized for the amplification of specific DNA fragments in conventional PCRs (
i.e., with Taq DNA Polymerase). However, the MgCl
2 Stock Solution may be used with other, more appropriate PCR buffers to optimize any type of PCR (
e.g., hot start PCR, high fidelity PCR, long template PCR).
General Description
The MgCl
2 Stock Solution is used for the individual adjustment of the Mg
2+-concentration in the PCR reaction and can be used in combination with the PCR buffer without MgCl
2. In most applications a concentration of 1.5mM MgCl
2 will yield satisfactory results with a dNTP concentration of 200μM each. In many cases, however, it is required to titrate the optimal Mg
2+ concentration to increase the specificity and the yield of the PCR reaction.
Biochem/physiol Actions
Magnesium (Mg2+) chelates nucleotide triphosphates (dNTPs) and its usage is one among the optimization strategies for polymerase chain reaction (PCR). It promotes orthophosphate synthesis from oligophosphates by stabilizing the phosphate groups of nucleotides.
