Uses
The reagent is designed for Flow Cytometry analysis. Suggested working dilution for Flow Cytometry is 5 μg/mL of sample. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.
General Description
CD9 (cluster of differentiation 9) is a human cell surface antigen. CD9 gene contains 8 exons extending over a length of 20kb on genomic DNA.The gene is mapped to human chromosome 2q12-pter. The encoded protein has a molecular weight of 24kDa and is expressed mainly on B-lineage-derived acute lymphoblastic leukemia cells and developing B lymphocytes.The protein is also present in blood platelets, neuroblastoma cell lines, normal and transformed epithelia, peripheral glia and neurons. CD9 antigen belongs to the transmembrane 4 superfamily (TM4SF) and is characterized with two external loops and four hydrophobic domains of membrane-spanning length.
Biochem/physiol Actions
Members of transmembrane 4 superfamily (TM4SF) including CD9 (cluster of differentiation 9) plays a vital role in cell proliferation, activation, motility and tumor cell metastasis. CD9 facilitates platelet activation and aggregation. The encoded protein also plays a major role in the amplification of a motorgenic signal in B cells includingβ1 integrins and the activation of protein tyrosine kinases. CD9 interacts with GM3 and downregulates tumor cell motility. CD9 and CD81 stimulate muscle cell fusion and aids in myotube maintenance.
