Uses
Duolink?proximity ligation assay(PLA?) allows for endogenous detection of protein interactions, post translational modifications, and protein expression levels at the single molecule level in fixed cells and tissue samples.
This product can be applied to both the
Duolink? In Situ Fluorescence Protocol and the
Duolink? In Situ Brightfield Protocol depending on the detection reagents used.
Visit our
Duolink? PLA Resource Center for information on how to run a Duolink
? experiment, applications, troubleshooting, and more.
To perform a complete Duolink
? PLA i
n situ experiment you will need two primary antibodies
(PLA, IHC, ICC or IF validated) that recognize two target epitopes. Other necessary reagents include a pair of PLA probes from different species (one PLUS and one MINUS), detection reagents, wash buffers, and mounting medium. Note that the primary antibodies must come from the same species as the Duolink
? PLA probes. Analysis is carried out using standard immunofluorescence assay equipment.HRP is also available for brightfield detection.
SpecificityPLA probe anti-Goat reacts with whole molecule goat IgG and the light chains of other goat immunoglobulin′s. The PLA probe anti-Goat may cross-react with sheep antibodies, but has minimal cross reactivity with chicken, guinea pig, Syrian hamster, horse, human, mouse,rabbit, and rat serum proteins. A PLUS probe of a different species must be used simultaneously with this product. See our
Product Selection Guide for more information.
Application NoteTwo primary antibodies raised in different species are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC) or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink
?PLA
in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.
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