Genopure PlasMid Midi Kit

Uses

The Genopure Plasmid Midi Kit prepares transfection-grade plasmid DNA in medium quantities (up to 100 μg plasmid) from bacterial cultures. Isolated plasmid is suitable for most molecular biology applications:

• Transfection
• Southern blotting
• Sequencing
• PCR
• Restriction analysis
• Cloning

General Description

Plasmid recovery was tested with 50 μg purified plasmid. The recovery was >90%, with more than 80% in supercoiled form. The yield of plasmid DNA was determined by isolating pBS from DH5a cells. From 30 mL culture volume with a density of A600 between 3 and 6, >85 μg of plasmid DNA was obtained.

The purity (checked by the ratio of A260/A280) is 1.8 + 0.2.

No RNA is detectable.

The kit components have been tested for the absence of nucleases according to current quality control procedures.