Uses
Deoxynucleotide set, 100 mM has been used in the amplification of DNA in polymerase chain reaction (PCR) and quantitative polymerase chain reaction (qPCR) techniques. It has also been used in multiplex PCR.
Uses
Deoxynucleotide Set, 100 mM has been used:
- to biotinylate λ-DNA for magnetic tweezers measurements
- in RAPD PCR
- in FISH probe generation and labeling
- in PCR amplification of genomic DNA
- in multiplex PCR
- in first-strand cDNA synthesis
- in TUNEL labeling
- in RT-PCR
General Description
Deoxynucleotide triphosphates (dNTPs) is highly essential to amplify DNA. This dNTP set contains 100mM solutions of individual dNTPs (dATP, dCTP, dGTP and TTP) in separate tubes for convenience and easy PCR reaction setup.
Biochem/physiol Actions
Deoxynucleotide triphosphates (dNTPs), the building blocks of nucleic acid molecules are acidic in nature. Optimal levels of dNTPs ensure proper chromosomal replication and in turn maintain genome stability.