General Description
Secreted alkaline phosphatase (SEAP) has become a powerful reporter gene for the investigation of promoter activity in transfected eukaryotic cells. The SEAP gene is cloned into vectors behind the promoter under investigation, then transfected into eukaryotic cells. The translated SEAP gene product is secreted from the cells, and is thus easily detected in a sample of culture medium without destroying cells and without time-consuming sample preparation. The elimination of cell lysis permits repeated sampling of the cell medium or other subsequent analysis. The SEAP Reporter Gene Assay, chemiluminescent is based on dioxetane CSPD (chloro-5-substituted adamantyl-1,2-dioxetane phosphate), and provides a convenient and highly sensitive method for the quantitation of transcriptional activity.
