Uses
The enzyme can be used without pre-activation for labeling water-soluble substances with reactive and accessible primary amino groups (
e.g., peptides or proteins) with peroxidase for use in analytical methods. It is particularly suitable for the coupling of antibodies (Ig, Ig Fab, and Ig F(ab′)
2 fragments from rabbit, mouse, sheep, and goat) with peroxidase. The resulting conjugate is optimal for use in immunochemical detection systems, for example, ELISA, immunohistochemistry, and immunoblotting procedures.
General Description
Peroxidase (POD) is an enzyme that includes a group of specific enzymes such as NAD peroxidase, NADP peroxidase, fattyacid peroxidase and a group of non specific enzymes from various sources. It is present in animals, higher plants and other organisms.
Biochem/physiol Actions
Peroxidase (POD) helps in the dehydrogenation of several organic compounds like phenols, aromatic amines, hydroquinones etc.
