Uses
Nick translation kit has been used in FISH (fluorescence
in situ hybridization) based labeling of tick and bacterial artificial chromosome. It has been used in labeling the probe (genomic DNA) in order to obtain GISH (genomic
in situ hybridization) signals.
General Description
Kit for radioactive or nonradioactive labeling of DNA by the nick translation method. This method utilizes a combination of DNase and DNA Polymerase to nick one strand of the DNA helix, then incorporates labeled nucleotides as the polymerase examines, or "proofreads" the nicked site.
