Uses
Anti-Guinea Pig IgG (whole molecule) Peroxidase antibody produced in rabbit has been used in diphtheria serology enzyme-linked immunosorbent assay (ELISA) and immunoblotting.
Uses
Anti-
H. pylori IgGs in guinea pig sera was measured by ELISA using HRP-conjugated goat anti-guinea pig IgG (A 7289) at a dilution of 1:2000 in 1% BSA/PBS for 1 hour at 37°C.
General Description
IgG consists of four sub-classes,?like?IgG1, IgG2, IgG3, and IgG4. IgG is composed of four polypeptide chains-two heavy chains (γ?chains)?and two light chains (κ or λ?chains)?which are linked by inter-chain?disulfide?bonds.?The heavy chains consist of a N-terminal variable domain (VH) and three constant domains (CH1, CH2, CH3). A hinge region exists between the CH1 and CH2 region. The light chains have one?N-terminal variable domain (VL) and?one?constant domain?(CL).
Biochem/physiol Actions
IgGs are glycoprotein antibodies that modulate several immune responses. It plays a major role in providing immunity to the infant from the mother via the placenta. IgG activates the complement system and regulates the antibody-dependent cell-mediated cytotoxicity (ADCC).
