Uses
The BM Chemiluminescence ELISA Substrate (POD) provides a substrate solution for peroxidase-based (POD, HRP) secondary detection systems with highly sensitive, enhanced chemiluminescence. This reagent has been optimized for ELISA applications to be run on a microplate chemiluminescence reader (96-well format) or tube-format luminometers.
General Description
The sensitivity of enzyme-linked immunosorbent assays often depends on the detection limit of the colorimetric substrates used. In addition, the dynamic range of a colorimetric assay is restricted to a maximum of two orders of magnitude due to the physical/chemical limitations of absorbance measurement (Law of Lambert & Beer). With chemiluminescence technology, a nonradioactive method, which combines the convenience of tube or microplate-based immunoassays with the advantages of isotopic assays, is now available. The BM Chemiluminescence ELISA Substrate (POD) has been evaluated with different immunoassays on the LB 96 P microplate reader and the LB 953 tube chemiluminescence analyzer from EG&G;Berthold.
