Description
Orexin B amide is a hypothalamic neuropeptide that regulates feeding behavior, wakefulness, and behavior under situations of high motivational relevance.1 It is an agonist of orexin (OX) receptors with IC50 values of 420 and 36 nM for human recombinant OX1 and OX2 receptors, respectively, expressed in CHO cells.2 It induces transient increases in intracellular calcium in CHO cells stably expressing OX1 and OX2 (EC50s = 2,500 and 60 nM, respectively) and in primary rat ventral tegmental area (VTA) dopaminergic neurons.2,3 Orexin B amide stimulates arachidonic acid (Item No. 90010) release from CHO cells expressing human recombinant OX1 (EC50 = 4.17 nM).4 It also stimulates food intake in freely feeding mice and goldfish when administered at intracerebroventricular doses of 30 nmol and 10 ng/g, respectively.2,5WARNING This product is not for human or veterinary use.
References
[1] STEPHEN V MAHLER. Motivational activation: a unifying hypothesis of orexin/hypocretin function[J]. Nature neuroscience, 2014, 17 10: 1298-1303. DOI:
10.1038/nn.3810[2] T SAKURAI. Orexins and orexin receptors: a family of hypothalamic neuropeptides and G protein-coupled receptors that regulate feeding behavior.[J]. Cell, 1998, 92 5: 1 page following 696. DOI:
10.1016/s0092-8674(02)09256-5[3] TOSHIAKI NAKAMURA . Orexin-induced hyperlocomotion and stereotypy are mediated by the dopaminergic system1[J]. Brain Research, 2000, 873 1: Pages 181-187. DOI:
10.1016/s0006-8993(00)02555-5[4] PAULI M TURUNEN. Arachidonic acid release mediated by OX1 orexin receptors[J]. British Journal of Pharmacology, 2010, 159 1: 212-221. DOI:
10.1111/j.1476-5381.2009.00535.x[5] HELENE VOLKOFF Richard E P Jennifer Marie Bjorklund. Stimulation of feeding behavior and food consumption in the goldfish, Carassius auratus, by orexin-A and orexin-B[J]. Brain Research, 1999, 846 2: Pages 204-209. DOI:
10.1016/s0006-8993(99)02052-1
