First Strand cDNA Synthesis Kit for RT-PCR (AMV) Price
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Sigma-Aldrich11483188001
30REACTIONS
$574
First Strand cDNA Synthesis Kit for RT-PCR (AMV) sufficient for 30?reactions (including 5 control reactions), kit of 1 (10 components), suitable for RT-PCR, hotstart: no, dNTPs included
First Strand cDNA Synthesis Kit for RT-PCR (AMV) Chemical Properties,Usage,Production
Uses
The First Strand cDNA Synthesis Kit for RT-PCR (AMV) is suitable for:
Detection of the presence or absence of RNA viruses or other RNA-containing microorganisms (in combination with PCR)
Quantification of mRNA for monitoring differential expression of a specific mRNA
First step in the "differential display of mRNA"
Generation of cDNA libraries with large and full-length inserts
Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) for reverse transcription of RNA into complementary DNA.
General Description
The use of AMV RT for first-strand cDNA synthesis has certain advantages. The higher thermostability of this enzyme compared to M-MuLV RT allows the reaction to be performed at +42°C, giving higher specificity and better resolution of secondary structures compared to a reaction performed at +37°C.
Preparation Products And Raw materials
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