Uses
Protector RNase Inhibitor may be used to:
- Protect mRNA in cDNA synthesis reactions, RT-PCR (in conventional thermal cyclers and qPCR systems), e.g., with the LightCycler? Instruments, in vitro transcription/translation system, RNase Protection Assay, in vitro RNA synthesis
- Protect viral RNA during in vitro virus replication
- Inhibit RNases during RNA isolation and purification
- Help prepare RNase-free antibodies
- Synthesize RNA probes for in situ hybridization
It is useful in any application where RNases could be a potential problem.
General Description
Protector RNase Inhibitor inactivates a wide spectrum of RNases, including
Thus, Protector RNase Inhibitor can help prevent RNase degradation in any application where RNases could cause problems. For instance, it can:
- protect mRNA during cDNA synthesis reactions, RT-PCR (in conventional thermal cyclers and qPCR systems), or in vitro transcription/translation reactions
- protect viral RNA during in vitro virus replication
- inhibit RNases during RNA isolation and purification
- be used in RNase protection assays
- help prepare RNase-free antibodies
Note: Protector RNase Inhibitor does not interfere with enzymes commonly used to prepare or analyze RNA.
