Description
(R)-Bromoenol lactone-d7 ((R)-BEL-d7) is intended for use as an internal standard for the quantification of (R)-BEL by GC- or LC-MS. The phospholipases are an extensive family of lipid hydrolases that function in cell signaling, digestion, membrane remodeling, and as venom components.1 The calcium-independent phospholipase A2 (iPLA2) are a PLA2 subfamily closely associated with the release of arachidonic acid in response to physiologic stimuli. (R)-Bromoenol lactone ((R)-BEL) is an irreversible, chiral, mechanism-based inhibitor of iPLA2γ. Unlike (S)-BEL, (R)-BEL does not inhibit iPLA2β except at high doses of 20-30 µM.2 (R)-BEL inhibits human recombinant iPLA2γ with an IC50 of approximately 0.6 µM.WARNING This product is not for human or veterinary use.
References
[1] SCHEUER W. Phospholipase A2–regulation and inhibition.[J]. Klinische Wochenschrift, 1989, 67 3: 153-159. DOI:
10.1007/bf01711343[2] C. M. JENKINS. Identification of Calcium-independent Phospholipase A2 (iPLA2) β, and Not iPLA2γ, as the Mediator of Arginine Vasopressin-induced Arachidonic Acid Release in A-10 Smooth Muscle Cells[J]. The Journal of Biological Chemistry, 2002, 26 1: 32807-32814. DOI:
10.1074/jbc.m202568200
