Uses
Tryptose Phosphate Broth has been used as a supplement:
- in Eagle′s minimum essential medium alpha for growing Vero cells
- for Vero-CCL81 cells in porcine epidemic diarrhea coronavirus propagation
- in Dulbecco modified Eagle medium in LR7cells for mouse hepatitis virus propagation
- in Glasgow minimum essential medium (GMEM) for Madin-Darby canine kidney (MDCK) cells.
General Description
Tryptose Phosphate Broth (TPB) comprises pyrimidine based components and is crucial for pyrimidine synthesis and electron transport chain in cells. In chick embryo fibroblast, it alleviates chloramphenicol inhibition.
Biochem/physiol Actions
Tryptose Phosphate Broth provides a source of amino acid based nutrients and survival factors that support the growth of fastidious micro-organisms such as Brucella, Streptococcus, and Neisseria; as well as eukaryotic cells such as insect and animal cells. Dextrose provides a fermentable carbohydrate that can be used by fastidious micro-organisms. Sodium chloride maintains the osmotic and ionic equilibrium and disodium phosphate provides the basic buffering capacity.
