Description
Geldanamycin-FITC is a novel geldanamycin fluorescent probe1?that may be used in a fluorescence polarization assay for HSP-90 inhibitors.2,3?May also be used for detection of cell surface HSP-90 and for other applications. Fluorescent geldanamycin probes have been used to characterize geldanamycin drug-target interactions for effective translation of?in vitro?pharmacology to cellular and?in vivo?efficacy.4
References
1) Llauger-Bufi?et al. (2003),?Synthesis of novel fluorescent probes for the molecular chaperone Hsp90;? Bioorg. Med. Chem. Lett.,?13?3975
2) Kim?et al. (2004),?Development of a fluorescent polarization assay for the molecular chaperone Hsp90;? J. Biomol. Screen.,?9?375
3) Austin?et al. (2012),?Fragment screening using capillary electrophoresis (Defrag) for hit identification of heat shock protein 90 ATPase inhibitors;? J. Biomol. Screen.,?17?868
4) Gooljarsingh?et al. (2006),?A biochemical rationale for the anticancer effects of Hsp90 inhibitors: slow, tight binding inhibition by geldanamycin and its analogues;? Proc. Natl. Acad. Sci. USA,?103?7625
