General Description
The Mouse IL-1β ELISA (enzyme-linked immunosorbent assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IL-1β in serum, plasma, cell culture supernatants and urine. Interleukin-1β (IL-1β) cytokine is generated by monocytes, dendritic cells, macrophages and keratinocytes. Pro-IL-1β, an IL-1β precursor undergoes proteolytic cleavage to produce its active form of 17?kDa. IL-1β is a potent inflammatory cytokine, and mediates inflammation through proinflammatory molecules: cyclooxygenase-2 (COX2) and nitric oxide. IL-1β release is stimulated by injury and increased levels are observed in response to severe pain. It has its main function in T-cell activation, antigen recognition and T-helper cell 17 cell development. IL-1β is also responsible for the formation of IL-17 and IL-22 by nature killer T (NKT) cells and NK cells. It is known to induce inflammation in psoriasis and rheumatoid arthritis. IL-1β controls intercellular adhesion molecule-1 expression. It is significantly associated with bone damage and vascular injury.
Biological Activity
Interleukin 1 β (IL-1β) stimulates the production of endogenous 92 kDa gelatinase (matrix metalloproteinases 9 (MMP-9)) and tissue inhibitors of MMP (TIMP -1). It is a proinflammatory cytokine th at induces various acute and chronic inflammation. Therefore, IL-1β can be considered as a potential therapeutic target for disease associated with fibrosis and tissue remodeling. IL-1β brings neutrophil and macrophage to the site of infection. Over expression of IL-1β in synovial fluid contributes to the pathogenesis of synovitis and degenerative changes of the cartilaginous tissue and bone of the temporomandibular joint. Mutations in IL-1β gene leads to renal manifestations and renal sequelae in Henoch-Schonlein purpura (HSP).
