Description
Autophagy-related 5 (ATG5), formerly known as apoptosis-specific protein (ASP), is a protein that is essential to autophagosome elongation. ATG5 is covalently conjugated to the C-terminal glycine residue of ATG12 (ATG12-ATG5) and forms a non-covalent complex with ATG16 (ATG12-ATG5-ATG16), which functions as an E3 ubiquitin ligase-like enzyme to facilitate LC3 transfer from ATG3 to phosphatidylethanolamine in canonical autophagy. ATG12-ATG5 also binds to the ATG12-ATG5-interaction region of the lysosomally localized protein TECPR1, freeing the TECPR1 pleckstrin homology domain to interact with phosphatidylinositol 3-phosphate components in the autophagosome membrane, promoting autophagosome-lysosome fusion. Polymorphisms in
ATG5 have been associated with various autoimmune diseases, including lupus nephritis and Behçet''s disease, gastrointestinal and colorectal cancers, as well as sporadic Parkinson''s disease and childhood asthma. Cayman’s ATG5 Monoclonal Antibody (Clone 4D5) can be used for ELISA, immunohistochemistry, and Western blot applications. The antibody recognizes the ATG5-ATG12 complex at approximately 56 kDa from human samples.
References
[1] CHINATSU OTOMO. Structure of the human ATG12ATG5 conjugate required for LC3 lipidation in autophagy[J]. Nature Structural & Molecular Biology, 2012, 20 1: 59-66. DOI:
10.1038/nsmb.2431[2] JUN HOE KIM. Insights into autophagosome maturation revealed by the structures of ATG5 with its interacting partners.[J]. Autophagy, 2015: 75-87. DOI:
10.4161/15548627.2014.984276[3] XIN YE Hong Z Xu Jie Zhou. Exploring the Role of Autophagy-Related Gene 5 (ATG5) Yields Important Insights Into Autophagy in Autoimmune/Autoinflammatory Diseases.[J]. Frontiers in Immunology, 2018: 2334. DOI:
10.3389/fimmu.2018.02334
