INSULIN, ZINC, BOVINE

Originator

Lente Insulin,Squibb,US,1971

Uses

Antidiabetic.

Manufacturing Process

First, a series of stock solutions are made.
Stock Solution 1: 2.18 g of recrystallized insulin are dissolved in 25 ml of 0.1 N hydrochloric acid, and distilled water to a volume of 125 ml is added.
Stock Solution 2: To 20 ml of an aqueous zinc chloride solution containing 1% zinc is added distilled water to a volume of 125 ml.
Stock Solution 3: 1.36 g of sodium acetate with 3 mols crystal water are dissolved in distilled water to a volume of 100 ml.
Then, 1.3 ml of glycerine are mixed with 0.5 ml of a 25% solution of methyl p-hydroxybenzoate in ethanol, and 50 ml of distilled water are added. To the produced mixture are, after sterile filtration, added 10 ml of the stock solution 1, 2.5 ml of the stock solution 2 and 10 ml of the stock solution 3, after which 3.0 ml of sterile 0.1 N sodium hydroxide are added, and the mixture is filled up with sterile distilled water to a volume of 100 ml. The insulin will be precipitated amorphously by the admixture of the sodium hydroxide, and the produced suspension acquires the pH value of 7. It will contain approximately 1 gamma zinc per insulin unit.

brand name

Semilente Iletin (Lilly); Semilente (Novo Nordisk).

Therapeutic Function

Hypoglycemic