ANTI-PHOSPHOTYROSINE

Uses

Anti-Phosphotyrosine antibody is suitable for use in indirect immunofluorescence (3-6 μg/mL using A431 stiμLated by human EGF) and immunoblot (using U2OS Dr1 cells ). The antibody may also be used for immunoprecipitation (1:40 using r at lumbar and thoracic lysates) and immunocytochemistry.

Biological Activity

Protein phosphorylation is the most abundant among post-translational modifications of cellular proteins, regulating intracellular signal transduction pathways in every living cell. Tyrosine, serine and threonine are the major amino acids th at are phosphorylated in proteins. Antibodies specific for phosphotyrosine are essential tools for the characterization of tyrosine phosphorylation in many signal transduction pathways.