Uses
3,3′-Diaminobenzidine (DAB) Liquid Substrate System tetrahydrochloride has been used as a substrate:
- in the immunoblot assay carried out to identify the changes in heat shock proteins (Hsps) expressed in the liver
- for visualization of sections after immunostaining
- in immunohistochemistry of mice knee joint sections
- in the immunolabeling of kisspeptin neurons in the preoptic area of camel
It produces a brown precipitate with peroxidase for use in immunohistological and immunoblotting staining procedures. Not recommended for ELISA (microwell) procedures.
General Description
3,3′-Diaminobenzidine (DAB) is a chromogenic substrate for peroxidase enzyme and is useful as a staining agent in immunohistological and immunoblotting techniques. The end product is resistant to alcohol therefore, a variety of counterstains and mounting media can be used with the DAB Liquid Substrate System. The DAB Liquid Substrate System provides all the chromogen and buffer/peroxide solutions needed to produce a fast and convenient DAB substrate solution..
