Uses
The PCR Master can be used instead of the single reagent Taq DNA Polmerase, or instead of the PCR Core Kit for nearly all simple, routine polymerase chain reaction applications. The only limitation is that the sample volume must not exceed half the total reaction volume. The PCR master mix is used for:
- Routine PCR
- RT-PCR
- Other primer-extension reactions, such as sequencing and labeling
General Description
The PCR Master contains all the reagents required to perform a standard PCR. All that must be added is the template DNA, the primers, and the required amount of water. The PCR Master contains a fixed MgCl
2 concentration of 1.5 mM. However, higher concentrations may be achieved by adding additional MgCl
2. PCR products generated by PCR Master are 3′-A single-overhang products. Therefore T/A cloning can be applied.
The PCR Master is stable at +2 to +8 °C, allowing immediate PCR without the time-consuming thawing of reagents.
