Uses
Anti-Human IgG (Fc specific)-Alkaline Phosphatase antibody is suitable for use in ELISA and western blot at 1:5000 dilutions. The product can also be used for dot blot (1:40,000) and immunohistochemistry (1:150 using formalin-fixed, paraffin-embedded sections).
Anti-Human IgG (Fc specific)-Alkaline Phosphatase antibody has been used in
- quantitative sandwich ELISA
- α-GalC ELISA
- ELISA assays
- ELISAs for IgG subclasses
General Description
Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. IgG is usually found as a monomer. IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. About 70 percent of the total immunoglobulin consists of IgG. Immunoglobulin G (IgG) participates in hypersensitivity type II and type III. Anti-Human IgG (Fc specific)-Alkaline Phosphatase antibody is specific for human IgG and human IgG, Fc fragment. The product does not react with human IgG, Fab fragment, light chains, IgA, and IgM. Additionally, the antibody does not bind to mouse and rat IgG. Goat anti-human IgG is conjugated to alkaline phosphatase by protein cross linking with 0.2% glutaraldehyde.
Biochem/physiol Actions
Immunoglobulin G (IgG) regulates immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders. Immunoglobulin G (IgG) mainly participates in hypersensitivity type II and type III.
