Uses
ExtrAvidin
?-Peroxidase is suitable for the following applications:
- Dot blot at a minimum dilution of 1:4,000
- Immunohistochemistry (using formalin-fixed, paraffin-embedded sections at a dilution of 1:100)
- Direct ELISA at a minimum dilution of 1:2,000
It has also been used for:
- Immunoprecipitation and Immunobloting
- Ra2I solid-phase binding assays
- Southwestern blotting
- Western blotting
- Immunocytochemistry
Uses
ExtrAvidin
?-Cy3
? is suitable for the following applications:
- Immunohistochemistry (formalin-fixed, paraffin-embedded sections) at a dilution of 1:100
- Immunocytochemistry.
- Used for labeling.
General Description
Avidin is a tetrameric or dimeric biotin-binding protein produced in the oviducts of birds, reptiles and amphibians and deposited in the whites of their eggs. It consists of four high affinity binding sites for biotin. ExtrAvidin is prepared from egg white avidin. It is a modified form of affinity purified avidin and combines high specific activity of avidin with the low background staining of streptavidin. It is a biotin binding protein produced by the bacteria
Streptomyces avidinii. ExtrAvidin has been conjugated to fluorescein isothiocyanate (FITC) and is used for various techniques.
Biochem/physiol Actions
ExtrAvidin? is prepared from egg white avidin. It is a tetrameric protein containing four high affinity binding sites for biotin. It combines the high specific activity of avidin with the low background staining of streptavidin, a biotin binding protein produced by the bacteria Streptomyces avidinii. It binds biotin with the high affinity of egg white avidin, however, it does not exhibit the unwanted non-specific binding reported for egg white avidin at physiological pH, such as the staining of mast cells.
