PRDX6 antibody

Source

Rabbit

Applications

PRDX6 antibody has been tested by ELISA, Western blot analysis, ICC/IF and Flow cytometry to assure specificity and reactivity.

Reactivity

Human;Mouse;Rat

Immunogen

Anti-human PRDX6 mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human PRDX6 amino acids 1-224 purified from E. coli.

Background

Peroxiredoxin 6 (PRDX6) belongs to the thiol-specific antioxidant protein family. PRDX6 is a bifunctional enzyme with 2 distinct active sites. PRDX6 is involved in redox regulation of the cell and can reduce Hydrogen peroxide and short chain organic, fatty acid, and phospholipid hydroperoxides. PRDX6 may have a role in the regulation of phospholipid turnover as well as in protection against oxidative injury. Furthermore, PRDX6 eases the oxidative stress and TGF-beta-induced abnormalities of human trabecular meshwork cells. In addition, PRDX6 is necessary for blood vessel integrity in injured skin.
At acidic pH, PRDX6 binds to reduced phospholipids, however at cytosolic pH PRDX6 binds only to phospholipids that are oxidized which is compatible with the role for PRDX6 in the repair of peroxidized cell membranes. Hydrogen peroxide-mediated hyperoxidation of PRDX6 induces cell cycle arrest at the G2/M transition via up-regulation of iPLA2 activity. Overexpression of PRDX6 is linked to oligodendroglioma.

References

[1] Kang, S.W. et al. (1998) J Biol Chem 273, 6303-11.
[2] Chen, J.W. et al. (2000) J Biol Chem 275, 28421-7.
[3] Manevich, Y. and Fisher, A.B. (2005) Free Radic Biol Med 38, 1422-32.
[4] Schremmer, B. et al. (2007) Subcell Biochem 44, 317-44.
[5] Lee, S.B. et al. (2009) Mol Cells 28, 583-8.
[6] Ho, J.N. et al. (2010) Mol Cancer Ther 9, 825-32.
[7] Yun, H.M. et al. (2014) Free Radic Biol Med 69, 367-76.
[8] Pak, J.H. et al. (2011) Cancer Invest 29, 21-8.
[9] Kalinina, E.V. et al. (2012) Bull Exp Biol Med 153, 878-81.
[10] Chatterjee, S. et al. (2011) J Biol Chem 286, 11696-706.