Uses
Vitamin B1 Monophosphate Chloride is a precursor of thiamine pyrophosphate (T344070), a thiamine (T344185) derivative produced by enzyme thiamine pyrophosphatase. Thiamine pyrophosphate is a cofactor used to catalyze various biochemical reactions.
Purification Methods
Purify it by recrystallisation from aqueous HCl, EtOH slightly acidified with HCl, EtOH/Me2CO, H2O, or H2O/EtOH/Et2O. Dissolve it in a small volume of H2O and mix it with EtOH/Me2CO (1:1) to give the HCl.H2O as crystals. Filter it off, wash it with Et2O and dry it in a vacuum. The chloride hydrochloride, m 215-217o(dec) is obtained when it is crystallised from aqueous HCl. [Wenz et al. Justus Liebigs Ann Chem 618 2280 1958, Viscontini et al. Helv Chim Acta 34 1388 1951, Leichssenring & Schmidt Chem Ber 95 767 1962, McCormick & Wright Methods Enzymol 18A 141, 147 1970, Beilstein 27 III/IV 1766.]
