STAT1 ANTIBODY

Source

Rabbit

Applications

The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Recommended dilution range for Western blot analysis is 1:500 ~ 1:1000. Recommended starting dilution is 1:500.

Reactivity

Human;Mouse;Rat;Monkey

Immunogen

Anti-human STAT1 mAb, clone PAT1F4A, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human STAT1 protein 1-712 amino acids purified from E. coli.

Background

STAT1 is a member of the Signal Transducers and Activators of Transcriptionfamily of transcription factors. STAT1 is involved in upregulating genes due to a signal by either type Ior type IIinterferons. In response to IFN-?stimulation, STAT1 forms homodimers or heterodimers with STAT3that bind to the GAS (Interferon-Gamma Activated Sequence) promoter element; in response to either IFN-? or IFN-?stimulation, STAT1 forms a heterodimer with STAT2that can bind the ISRE (Interferon Stimulated Response Element) promoter element. In either case, binding of the promoter element leads to an increased expression of ISG (Interferon Stimulated Genes).

References

[1] Heim, M.H. (1999) J Recept Signal Transduct Res 19, 75-120.
[2] Durbin, J.E. et al. (1996) Cell 84, 443-50.
[3] Meraz, M.A. et al. (1996) Cell 84, 431-42.
[4] Ihle, J.N. et al. (1994) Trends Biochem Sci 19, 222-7.
[5] Frank, D.A. (1999) Mol Med 5, 432-56.
[6] Wen, Z. et al. (1995) Cell 82, 241-50.