Uses
The High Pure RNA Tissue Kit rapidly isolates total RNA from mammalian tissues such as mouse liver, spleen, lung, and heart. The isolated RNA can be used in many downstream applications:
- RT-PCR
- Northern blotting
- RACE
- Primer extension
- Differential display
- cDNA library construction
- RNase protection assay
- In vitro translation
General Description
The High Pure RNA Tissue Kit is designed for the purification of total, intact RNA from tissue samples, free of any contaminating DNA. Low to medium throughput RNA isolation. Nucleic acids bind to the surface of the glass fleece in the presence of a chaotropic salt (guanidine HCl). This allows the High Pure Filter Tube to specifically immobilize nucleic acids (both DNA and RNA) while they are freed of contaminants. For RNA isolation, the binding conditions can be optimized to ensure immobilization of all the RNA. High pure RNA isolation kit can rapidly isolate intact total RNA from a broad range of research sample materials, including cultured cells, mammalian blood, white blood cells (WBCs), yeast, and bacteria. It rapidly isolates total RNA from mammalian tissues such as mouse liver, spleen, lung, and heart.
Capacity: The High Pure Spin Filter Tubes hold up to 700 μL sample volume.
Sample Material: Solid tissue (e.g., mouse liver, spleen, lung, heart): 1 - 25 mg.
Note: Sample size depends on the method used to prepare the tissue; larger samples (>10 mg) should be processed via rotor-stator homogenization.
