Manufacturing Process
Bromoacetylation of N-acetylcytidine with 2-acetoxy-2-methylpropanoyl
bromide
A 5 L three-nicked, round-bottomed flask equipped with a mechanical stirrer,
thermometer, nitrogen inlet tube, and additional funnel was charge with 142.6
g (0.5 mole) of N-acetylcytidine, and 1.25 L of acetonitrile. The suspension
was stirred under nitrogen, cooled to 5°C (ice-bath), and treated dropwise
(during 20 min) with 225 ml of 2-acetoxy-2-methylpropanoyl bromide (AIBB)
during 30 minutes. At the completion of the addition, a homogeneous solution
resulted. It was stirred at room temperature overnight (the reaction was
complete within 3 hr), cooled to 5°C, and diluted with 1.25 L of ethyl acetate.
After recooling to 5°C, 2.0 L of saturated sodium bicarbonate was added. The
mixture was stirred for 5 minutes, the organic phase was separated, and the
aqueous phase was back-extracted with 500 ml of ethyl acetate. The
combined organic extracts were washed with 1 L of saturated brine, dried
(MgSO 4 ), and evaporated to give a gum. Final drying at 40°C (1 mm) for 1 hrgave 264.7 g (102%) of a white solid. High pressure liquid chromatographic
analysis gave the following results (major peaks only): 40% of [2R-
[2α,3β,4α,5α(S*)]]-N-[1-[3-(acetyloxy)-5-[(2-(acetyloxy)-1-oxopropoxy]
methyl]-4-bromotetrahydro-2-furanyl]-1,2-dihydro-2-oxo-4-pyrimidinyl]
acetamide (a) and 24% of its regioisomer (b).
Preparation of [2R-[2α,3β,4α,5α(S*)]]-N-[1-[3-(acetyloxy)-5-[(2-(acetyloxy)-
1-oxopropoxy]methyl]-4-bromotetrahydro-2-furanyl]-1,2-dihydro-2-oxo-4-
pyrimidinyl]acetamide (a) and its regioisomer (b).
A 1-L, three-necked, round-bottomed flask equipped with a mechanical stirrer
and argon inlet was charged with 28.52 g of N-acetylcytidine in 250 ml of
acetonitrile. The mixture was cooled to 10°C and treated with 48.75 g of (S)-
(-)-2-acetoxypropionyl bromide during 15 minutes. It was stirred at room
temperature overnight, cooled to 10°C, treated with 400 ml of cold (0°C)
saturated sodium bicarbonate, and extracted with 250 ml of ethyl acetate. The
extract was washed with 200 ml of saturated brine, dried (MgSO 4 ) and
evaporated to give 45.45 g of a white foam. Reversed phase chromatography
(C 18 column) with 40% methanol in water gave a pure sample of (a).
Zinc-copper couple was prepared by the next way:
A 12 L three-necked, round-bottomed flask equipped with a mechanical stirrer
was charged with 4.50 kg of zinc dust. The zinc dust was washed with 3.75 L
of 3% aqueous hydrochloric acid by stirring for 3 to 5 minutes. The
hydrochloric acid was decanted from the solid. This cycle was repeated with
3x3.75 L of 3% hydrochloric acid. The reaction was slightly exothermic and
the volume of the zinc dust increased to double its original volume. The zinc
dust was then washed with 4x3.0 L of deionized water to remove any residual
hydrochloric acid. After all the water was decanted, the spongy zinc layer was
treated with a solution made by dissolving 240.0 g of cupric sulfate dihydrate
in 7.5 L of deionized water. The suspension was stirred rapidly as the solution
was added. The aquamarine color of the cupric sulfate solution was removed
almost immediately and the zinc suspension changed in color from gray to
black. The near colorless aqueous layer was decanted and the solid was
washed with 4x3.0 L of deionized water. The suspension of zinc-copper couple
was filtered through a piece of Whatman No. 1 filter paper, then washed with
4x30 L ethanol and 3x3.0 L of ether. The solid was carefully dried at 25°C and
140 mm overnight to remove ether, then for 3 hr at 130°-140°C (0.5 mm).
The solid was cooled to room temperature under vacuum and was stored
under argon in amber bottles. The procedure yielded 3.84 kg of zinc-copper
couple.
Preparation of [2R-[2α,5α(S*)]]-N-[1-[5-[[2-(acetyloxy)-1-oxopropoxy]
methyl]-2-5-dihydro-2-furanyl]-1,2-dihydro-2-oxo-4-pyrimidinyl]acetamide.
A total of 1.47 g of a mixture of bromoacetates in acetonitril was reduced with
800 mg of zinc-copper couple. The mixture was stirred under argon at room
temperature overnight. The mixture was deoxygenated by evacuation followed
by filling the reaction vessel with argon (oxygen-free nitrogen may be used);
this procedure was repeated three times. It was filtered over Celite, the flask
was rinsed out with of acetonitrile, and the rinse was used to wash the Celite.
The combined filtrate and washing were evaporated (40°C), and the residue
was dissolved in of methylene chloride. This was added to a previously prepared solution of ethylenediaminetetraacetic acid disodium salt dihydrate
(Fluka) in deionized water containing of sodium bicarbonate. The mixture was
stirred vigorously for 1.5 hr, and filtered over Celite, which was washed with
methylene chloride. The organic phase was separated and the aqueous phase
was re-extracted with of methylene chloride. The combined organic was
washed with of saturated sodium bicarbonate, which was back-extracted with
of methylene chloride. The combined organic was dried (MgSO 4 ), filtered, and
concentrated. To this was added of acetic anhydride followed by 40 g of poly-
4-vinylpyridine, and the mixture was stirred under nitrogen for 3 hr. It was
filtered over Celite, which was washed with methylene chloride. The combined
filtrate and washing were evaporated, toluene was added, and the mixture
was evaporated again, ether was added with vigorous stirring for 15 minutes.
The mixture was filtered (some scraping of the flask was necessary) and
washed with ether to give 570 mg of after crystallization from hot
tetrahydrofuran, melting point 125°C; [α] D 25 +119.04°(c=0.25, CHCl 3 ).
Preparation of [2R-[2α,5α(S*)]]-N-[1-[5-[[2-(acetyloxy)-1-oxopropoxy]
methyl]tetrahydro-2-furanyl]-1,2-dihydro-2-oxo-4-pyrimidinyl]acetamide.
A solution of 720 mg of 2R-[2α,5α(S*)]]-N-[1-[5-[[2-(acetyloxy)-1-
oxopropoxy]methyl]-2-5-dihydro-2-furanyl]-1,2-dihydro-2-oxo-4-
pyrimidinyl]acetamide set forth in 10 ml L of methanol and 10 ml of
tetrahydrofuran was hydrogenated over 200 mg of 10% palladium on charcoal
at room temperature and atmospheric pressure until hydrogen uptake ceased
(10 ml). The mixture was filtered over Celite and the filtrate was evaporated
to give a gum. Chromatography on 10 g of silica (70-230 mesh) with 10%
methanol in methylene chloride, gave 290 mg of the product as a foam,
[α] D 25 +88.43° (C=0.99, CHCl 3 ).
Preparation of 2',3'-dideoxycytidine.
A solution of 20.7 g of [2R-[2α,5α(S*)]]-N-[1-[5-[[2-(acetyloxy)-1-
oxopropoxy]methyl]tetrahydro-2-furanyl]-1,2-dihydro-2-oxo-4-pyrimidinyl]
acetamide in 100 ml of ethanol was treated with 10.0 ml of Triton B (N-
benzyltrimethyl-ammonium hydroxide), and the mixture was stirred at room
temperature overnight. The mixture was concentrated to 20 ml, cooled to
0°C, and the product was collected by filtration. It was washed with 10 ml of
cold ethanol to give 4.48 g of 2',3'-dideoxycytidine, melting point 215°-218°C,
as an white solid.
General Description
Zalcitabine, 2',3'-dideoxycytidine or ddCyd, is an analog ofcytosine that demonstrates activity against HIV-1 and HIV-2,including strains resistant to AZT. The potency (in peripheralblood mononuclear cells) is similar to that of AZT, but thedrug is more active in populations of monocytes andmacrophages as well as in resting cells.
The oral bioavailability of zalcitabine is over 80% in adultsand less in children.The major dose-limiting side effect isperipheral neuropathy, characterized by pain, paresthesias,and hypesthesia, beginning in the distal lower extremities.These side effects are typically evident after several months oftherapy with zalcitabine. A potentially fatal pancreatitis is anothertoxic effect of treatment with ddC. The drug has beenapproved for the treatment of HIV infection in adults with advanceddisease who are intolerant to AZT or who have diseaseprogression while receiving AZT. ddC is combined with AZTfor the treatment of advanced HIV infection.
