Chemical Properties
Appearance: Orange solid
ex/em: 484/504nm (PBS/ethanol mixture)
Extinction coefficient (ε): 150000 Lmol1cm1
Quantum yield (Φ): 0.12
Uses
Cyanines are formally compounds with two nitrogen atoms linked by an odd number of methene units. 26 28 The nitrogen atoms are parts of the heterocyclic units (such as indole, benzoxazol, or benzothiazol) . The structures and optical properties of representative cyanine dyes used for in vivo imaging are presented[1]. Cyanines are characterized by long wavelength, tunable absorption and emission, very high extinction coefficient (up to 300,000 M 1 cm 1), good water solubility, and relatively straightforward synthesis[2].
References
[1] Altman RB, et al. Cyanine fluorophore derivatives with enhanced photostability. Nat Methods. 2011 Nov 13;9(1):68-71. DOI:
10.1038/nmeth.1774[2] Shindy, H. A. (2017). Fundamentals in the chemistry of cyanine dyes: A review. Dyes and Pigments, 145, 505–513. doi:10.1016/j.dyepig.2017.06.029
