Uses
Duolink?proximity ligation assay(PLA?) allows for endogenous detection of protein interactions, post translational modifications, and protein expression levels at the single molecule level in fixed cells and tissue samples.
Follow the
Duolink? In Situ Fluorescence Protocol to use this product. A set of
short instructionsis also available.
Visit our
Duolink? PLA Resource Center for information on how to run a Duolink
? experiment, applications, troubleshooting, and more.
To perform a complete Duolink
? PLA
in situ experiment you will need two primary antibodies
(PLA, IHC, ICC or IF validated) that recognize two target epitopes. Other necessary reagents include a pair of PLA probes from different species (one PLUS and one MINUS), detection reagents, wash buffers, and mounting medium. Note that the primary antibodies must come from the same species as the Duolink
? PLA probes. Analysis is carried out using standard immunofluorescence assay equipment.
SpecificityFarRed fluorescence detection reagents are often used with Cyanine 5 filter.
Application NoteTwo primary antibodies raised in different species are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC) or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink
? in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.
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? projects
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General Description
Duolink
? In Situ Detection Reagents Far Red contains all the necessary Duolink
In situ reagents to perform the amplification and detection of bound PLA
? probes. The detection probes contain a fluorophore (lex = 644nm and lem = 669nm), which may be visualized using the same filter as Cy
?5. Experiments conducted using Duolink
In Situ reagents can detect and visualize protein interactions, protein expression levels and post translational modifications at the single molecule level in fixed cells and tissue samples.
