Uses
Suggested starting dilutions are as follows: ICC/IF: 1:100-1:1000, IHC-P: 1:100-1:1000, WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Uses
All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project
(www.proteinatlas.org)and as a result, are supported by the most extensive characterization in the industry.
The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. To view these
protocols and other useful information about Prestige Antibodies and the HPA, visit .
General Description
Structural maintenance of chromosomes protein 6 (SCM6) belongs to the structural maintenance of chromosomes family. There are at least six SMC family members that form three heterodimers in specific combinations. SCM6 gene is mapped to human chromosome 2p24.2. Structurally, SCM6 has DNA-binding domains at the N and C termini, a long coiled-coil domain, and a hinge region.
Biochem/physiol Actions
Structural maintenance of chromosomes protein 6 (SCM6) along with SMC5 is mainly involved in the cellular response to DNA damage in yeast as well as humans. The yeast SMC5/6 complex contains several non-SMC elements (NSE), including methyl methanesulfonate-sensitivity protein 21 (MMS21) which sumoylates components of the complex. The SMC5/6 complex is recruited to nuclease-induced double strand breaks(DSBs). This event is crucial for human SMC1/3 cohesion complex recruitment to DSBs. SCM6 is known to interact with the papillomavirus E2 protein. Mitotic aberration and abnormal distribution of topoisomerase IIα are associated with depleted levels of Smc5 and Smc6.
