Description
LUN-15112 is used to probe RNA structure and conformation in live cells, using the cross-linking of matched RNAs and deep sequencing or COMRADES method. LUN-15112 crosslinks the RNA and the azide enables subsequent click reaction with biotin. The COMRADES method has been used to determine the architecture of the Zika virus RNA genome inside cells, and identified multiple site-specific interactions with human noncoding RNAs. It has also been used to map RNA-RNA interactions along the SARS-CoV-2 genome and subgenomes inside cells. LUN-15112 is cell permeable. This product has no formal name.
Uses
Psoralen-triepthylene glycol azide is a compound used to probe the structure and conformation of RNA in living cells, using matching RNA crosslinking and deep sequencing or comradery methods[1][2].
References
[1] Ziv O, et al. The Short- and Long-Range RNA-RNA Interactome of SARS-CoV-2. Mol Cell. 2020 Dec 17;80(6):1067-1077.e5. DOI:
10.1016/j.molcel.2020.11.004[2] Ziv O, et al. COMRADES determines in vivo RNA structures and interactions. Nat Methods. 2018 Oct;15(10):785-788. DOI:
10.1038/s41592-018-0121-0
