Description
Phaseolus vulgaris agglutinin is the name ascribed to a family of lectins, each of which consists of four subunits. There are two different types of subunits. One appears to be involved primarily in red cell agglutination and has been designated the “E” subunit (for erythroagglutinin). The other type is involved in lymphocyte agglutination and mitogenic activity and has been termed the “L” subunit (for leucoagglutinin). These subunits combine to produce five isolectins.
Biotinylated PHA-E has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium azide.
Chemical Properties
White freeze-dried powder, soluble in water, insoluble in organic solvents; divided into two components: PHA-P and PHA-M, with hemagglutination and serum precipitation effects.
Biological Activity
Phaseolus Vulgaris Erythroagglutinin (PHA-E) is a plant lectin that can be used as a probe to specifically bind biomolecules (such as polysaccharides, peptides, etc.).Phaseolus Vulgaris Erythroagglutinin (PHA-E) is a biological material or organic compound that can be used in life science research[1][2].
References
[1] SHANSHAN SHA. Phaseolus vulgaris Erythroagglutinin (PHA-E)-Positive Ceruloplasmin Acts as a Potential Biomarker in Pancreatic Cancer Diagnosis.[J]. ACS Applied Materials & Interfaces, 2022. DOI:
10.3390/cells11152453.
[2] G L NICOLSON S J S. Ferritin-conjugated plant agglutinins as specific saccharide stains for electron microscopy: application to saccharides bound to cell membranes.[J]. Proceedings of the National Academy of Sciences of the United States of America, 1971, 68 5: 942-945. DOI:
10.1073/pnas.68.5.942.[3] CHEN GAO. Extraction，Separation and Purification of Phaseolus vulgaris Agglutinin（PHA）from Common Bean Fresh Pods[J]. Journal of Jianghan University, 2016, 41 1: 496. DOI:
10.16389/J.CNKI.CN42-1737/N.2016.06.003.
