Description
DNA and RNA are damaged by oxidation during aging and in a variety of disease states including cancer. Guanine is the base is most prone to oxidation, and the repair processes initiated to correct the damage release multiple oxidized guanine species into the urine, including 8-hydroxyguanosine (8-OHG), 8-hydroxy-2’-deoxyguanosine (8-OHdG), and 8-hydroxyguanine. Some commercial vendors offer immunoassays that detect only 8-OHdG. Cayman''s DNA/RNA Oxidative Damage (Clone 7E6.9) ELISA Kit is a competitive assay that can be used to measure both 8-OHdG and 8-OHG. Since the antibody used in the assay recognizes damaged nucleic acid species other than 8-OHdG, the value obtained with our assay will be higher than that obtained by competitor ELISAs or by LC/MS analysis that measure a single species. As such, caution is recommended when comparing results obtained from this kit to those obtained by other methods.
References
[1] WEI GAN . Age-dependent increases in the oxidative damage of DNA, RNA, and their metabolites in normal and senescence-accelerated mice analyzed by LC–MS/MS: Urinary 8-oxoguanosine as a novel biomarker of aging[J]. Free Radical Biology and Medicine, 2012, 52 9: Pages 1700-1707. DOI:
10.1016/j.freeradbiomed.2012.02.016[2] FEI SHI. Oxidative damage of DNA, RNA and their metabolites in leukocytes, plasma and urine of Macaca mulatta: 8-oxoguanosine in urine is a useful marker for aging.[J]. Free Radical Research, 2012, 46 9: 1093-1098. DOI:
10.3109/10715762.2012.689428[3] K. ROSZKOWSKI R O. Urinary 8-oxoguanine as a predictor of survival in patients undergoing radiotherapy.[J]. Journal of Clinical Oncology, 2013, 5 3 1. DOI:
10.1200/jco.2013.31.15_suppl.e22197[4] S. YOON J K. Poly(ADP-ribose) polymerase 1 contributes to oxidative stress through downregulation of sirtuin 3 during cisplatin nephrotoxicity[J]. Anatomy & Cell Biology, 2016, 49 1: 165-176. DOI:
10.5115/acb.2016.49.3.165
