Uses
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
General Description
Aurora B (AIRK2, AIR-2 kinase, AIM-1) is a serine/threonine kinase. Aurora B is evolutionally conserved from yeast to human. The
Drosophila serine/threonine protein kinase Aurora and the
S. cerevisiae IpI1 kinase are highly homologous to human Aurora B.
Biochem/physiol Actions
Aurora B plays key roles in chromosome segregation, cytokinesis, and cancer development. It also plays a role in chromosomal condensation by phosphorylating the H3 histone. In C. elegans, Aurora-B is required for normal localization and function of the ZEN-4/CeMKLP (Kinesin like protein/mitotic kinesin-like protein), a kinesin-related protein essential for completion of cytokinesis. Loss of the Aurora B kinase results in chromosome segregation defects and failures in cytokinesis. Aurora B displays a localization pattern typical of chromosomal passenger proteins as the inner centromeric protein (INCENP), TD-60 and Survivin. INCENP and Survivin interact directly with Aurora B. Chromosomal passenger proteins undergo dynamic redistribution during mitosis. They localize at centromers during prometaphase and relocate to midzone microtubules and midbodies during anaphase and telophase. The mRNA and protein levels of Aurora B are induced during G2M and decrease rapidly after the end of mitosis. Levels of Aurora B are increased in several human cancer cell lines.
