Description
Isoprostanes are prostaglandin (PG)-
like products of free-
radical induced lipid peroxidation. Although the isoprostanes derived from arachidonic acid are the best characterized, many other polyunsaturated fatty acids can form isoprostanes. (±)5-iPF
2α-
VI is one of dozens of possible stereo-
and regioisomeric isoprostanes which can be formed from arachidonic acid. To date, the most extensively studied of these is 8-
isoprostane (8-
epi-
PGF
2α, iPF
2α-
III). However, 8-
isoprostane is a minor isoprostane constituent when compared to some of the other isomers which form in natural conditions of oxidative stress. (±)5-iPF
2α-
VI is an isoprostane from the unique Type VI class of isoprostanes. This class has been shown to be one of the major isoprostane products, in contrast to 8-
isoprostane. In addition to being produced in greater abundance than 8-
isoprostane, Type VI isoprostanes form internal lactones, which facilitates their extraction and purification from biological samples.
