Description
Polysucrose (sold as FicollTM by GE Healthcare) is a high molecular weight polymer formed by the copolymerization of sucrose with epichlorohydrin. It is used for cell separation and organelle isolation, in the study to develop a new method for the purification of human motile spermatozoa, and in the investigation of antiproliferative and antitumor activities of D-reverse peptides.
Chemical Properties
pH 7.0-8.0
Uses
Ficoll is a hydrophilic neutral highly branched polysaccharide use to establish density gradients to separate blood component cells and organelles. It is part of the separation medium called Ficoll-Paque.
Uses
Polysucrose has been used in a study to develop a new method for the purification of human motile spermatozoa. It has also been used in a study that investigated the antiproliferative and antitumor activities of D-reverse peptides.
Uses
Used for cell separation and organelle isolation.
Polysucrose has been used in a study to develop a new method for the purification of human motile spermatozoa. It has also been used in a study that investigated the antiproliferative and antitumor activities of D-reverse peptides.
General Description
Ficoll 400 is a highly branched polymer formed by the copolymerization of sucrose and epichlorohydrin. Ficoll 400 is completely non-ionic. Because of the abundance of hydroxyl groups, Ficoll 400 is very hydrophilic and extremely water-soluble. The most common application for Ficoll 400 is as a density gradient medium for the separation and isolation of eukaryotic cells, organelles and bacterial cells. Density ranges up to 1.2 g/ml can be attained. It has also been utilized in a variety of other applications.
