Description
1-Stearoyl-2-15(S)-HETE-sn-glycero-3-PE-d11 is intended for use as an internal standard for the quantification of 1-stearoyl-2-15(S)-HETE-sn-glycero-3-PE (Item No. 21139) by GC- or LC-MS. 1-Stearoyl-2-15(S)-HETE-sn-glycero-3-PE is a phospholipid that contains stearic acid (Item No. 10011298) at the sn-1 position and 15(S)-HETE (Item No. 34720) at the sn-2 position. It is formed in human peripheral monocytes activated by the calcium ionophore A23187 (Item No. 11016) by direct oxidation of 1-stearoyl-2-arachidonoyl-sn-glycero-3-PE (SAPE; Item No. 25871) by 15-lipoxygenase (15-LO).1,2 Phosphoethanolamine (PE) HETEs (PE-HETEs), including 1-stearoyl-2-15(S)-HETE-sn-glycero-3-PE, are the main source of esterified HETE in ionophore-activated monocytes.WARNING This product is not for human or veterinary use.
References
[1] BENJAMIN H MASKREY. Activated platelets and monocytes generate four hydroxyphosphatidylethanolamines via lipoxygenase.[J]. The Journal of Biological Chemistry, 2007, 282 28: 20151-20163. DOI:
10.1074/jbc.m611776200[2] ALWENA H MORGAN. Quantitative assays for esterified oxylipins generated by immune cells[J]. Nature Protocols, 2010, 5 12: 1919-1931. DOI:
10.1038/nprot.2010.162
