Description
20-
HETE-
d
6 contains six deuterium atoms at the 16, 16', 17, 17', 18 and 18' positions. It is intended for use as an internal standard for the quantification of 20-
HETE by GC- or LC-MS. 20-
HETE is a cytochrome P450 (CYP450) metabolite postulated to play an autacoid role in the renal and cerebral vasculature.
1 In rat cerebral microvessels, 20-
HETE is a vasoconstrictor that mediates pressure-
induced autoregulatory vasoconstriction.
2 20-
HETE is excreted mainly as the glucuronide conjugate. The concentration of free 20-
HETE (20-
40 pg/ml in human urine) is about 10-
fold lower than the corresponding concentration of the 20-
glucuronide.
3
References
1. McGiff, J.C., and Quilley, J.
20-HETE and the kidney: Resolution of old problems and new beginnings Am. J. Physiol. 277(3),R607-R623(1999).
2. Gebremedhin, D., Lange, A.R., Lowry, T.F., et al.
Production of 20-HETE and its role in autoregulation of cerebral blood flow Circ. Res. 87(1),60-65(2000).
3. Prakash, C., Zhang, J.Y., Falck, J.R., et al.
20-Hydroxyeicosatetraenoic acid is excreted as a glucuronide conjugate in human urine Biochem. Biophys. Res. Commun. 185(2),728-733(1992).
