Uses
Dicyanocobinamide has been used for the crystallization of vitamin B12-binding protein (BtuF). It has also been used for the destabilization of BtuCD-BtuF Interaction.', 'For use in determining non-intrinsic factor (R protein) in intrinsic factor preparations.
Biological Activity
Dicyanocobinamide (Cbi) functions as a soluble guanylate cyclase (sGC) co-activator and targets the N-terminal regulatory regions. This action is similar to the effect of forskolin on adenylyl cyclases. Dicyanocobinamide elevates the intracellular cyclic guanosine monophosphate (cGMP) levels and exhibits vasorelaxant activity in rats. It is used along with various cobalamins in studies of the inhibition of HIV-1 integrase and nitric oxide synthase.
