Description
Tetrazine (methyltetrazine) derivative of fluorescein, pure 5-isomer.
Tetrazines are reactive heterodiene components for [4+2] inverse electron demand Diels-Alder reaction (ied-DA) with strained cycloalkenes (trans-cyclooctenes, cyclopropenes). This reaction is also called TCO (tetrazine-cyclooctene) ligation. The reaction is among the fastest bioconjugation reactions.
Uses
6-Methyl-Tetrazine-5-FAM, is a Fluorescent dye, that can be used for various labeling applications in chemistry. (Abs/Em = 492/517 nm)