Uses
Anti-DNMT1 antibody produced in rabbit is suitable for microarray and immunoblotting at a working concentration of 0.5-1.0μg/mL using nuclear extracts of 293T or HeLa cells. It was used at 1:1000 working dilution for immunoblot analysis in a study to analyze whether some miRNAs are aberrantly expressed and target DNMT1 in NiS (nickel sulfide)-transformed cells. It was used for immunoprecipitation of DNMT1 from nuclear lysates of colon cancer cells transfected with DNMT1 vectors in a study. It was used as a primary antibody at a working dilution of 1:1000 for western blot analysis of total protein extracted from left murine testis tissue exposed to low-dose-rate radiation in a study.
General Description
In humans, the gene encodes a DNA (cytosine-5)-methyltransferase with 1616 amino acids. The N-terminal contains the regulatory domain, while the C-terminal region contains the catalytic domain. Two isoforms of DNMT1 have been isolated, DNMT1a and DNMT1b, the difference residing in 16 extra amino acids within the latter.
Biochem/physiol Actions
DNMT1 (DNA (cytosine-5-)-methyltransferase 1) is involved in the maintenance of methylation in somatic cells. During DNA methylation, DNMT1 helps to form the correct pattern of DNA strand by arranging heterochromatin. The CXXC region shows higher binding affinity for unmethylated DNA compare to the hemimethylated or methylated DNA. By binding to the CpG dinucleotide, it hypermethylates CpG islands as well as helps in silencing of tumor suppressor genes. Alteration in DNMT1 activity leads to various human diseases such as cancer and genetic disorders.
