Uses
2-Hydroxyoctanoic acid is a medium chain acyl-CoA synthetase inhibitor with a Ki of 500 μM[1].
Definition
ChEBI: A hydroxy fatty acid that is caprylic (octanoic) acid substituted by a hydroxy group at position 2.
Biological Activity
(±)-2-Hydroxyoctanoic acid inhibits purified medium-chain acyl-CoA synthetase from bovine liver mitochondria (Ki, 500 uM) with hexanoic acid as a substrate .', '(±)-2-Hydroxyoctanoic acid is a mixture of the D and L-2-hydroxyoctanoic acid enantiomers. (±)-2-Hydroxyoctanoic acid may be used in the development of medium-chain hydroxy-fatty acid enantiomer separation and detection systems. 2-Hydroxyoctanoic acid may be used as an inhibitor to study the kinteics of medium chain acyl-CoA synthetase(s).
Purification Methods
Crystallise the acid from EtOH/pet ether or ether/ligroin. [Beilstein 3 IV 873.]
References
[1] F Kasuya, et al. Inhibition of a medium chain acyl-CoA synthetase involved in glycine conjugation by carboxylic acids. Biochem Pharmacol. 1996 Nov 22;52(10):1643-6. DOI:
10.1016/s0006-2952(96)00563-1
