Description
Maresin conjugates in tissue regeneration 3 (MCTR3) is a specialized pro-resolving mediator (SPM) synthesized from docosahexaenoic acid (DHA; ) in macrophages.
1,2 DHA is oxidized to maresin 1 (MaR1; ), which is converted to MCTR1 by glutathione S-transferase Mu 4 or leukotriene C
4 synthase, then to MCTR2 by γ-glutamyl transferase, and to MCTR3 by dipeptidase.
3 MCTR3 accelerates tissue regeneration in planaria (1 and 100 nM) approximately as potently as MCTR2 and more potently than MCTR1.
2 Pretreatment with MCTR3 prior to
E. coli administration in mice reduces neutrophil infiltration, shortens the inflammatory resolution period, and increases phagocytosis of
E. coli by macrophages. When administered at a dose of 100 ng 12h post
E. coli infection in a mouse model of peritonitis, MCTR3 selectively reduces the amount of the eicosanoids PGD
2 , PGE
2 , PGF
2α , and TXB
2 in the exudate.
Uses
MCTR3 is a potent cytokine of pro-resolving mediating maresin conjugates in tissue regeneration (MCTR), which reduces the inflammatory response and promotes the tissue regeneration. MCTR3 exhibits potency in ameliorating LPS-induced acute lung injury and arthritis[1][2][3].
in vivo
MCTR3 (2 ng/g, i.p., single dose) reduces inflammation and oxidative response in the LPS-induced acute lung injury and decreases the apoptosis in C57BL/6 mice[1].
MCTR3 (1-100 nM, in cold water) stimulates tissue regeneration in surgical injuried Planaria in a dose-dependent manner with a 50% tissue regeneration TRI50 of 2.5 days[2].
| Animal Model: | Lipopolysaccharide induced acute lung injury in C57BL6 mice[1] |
| Dosage: | 2 ng/g |
| Administration: | i.p., single dose |
| Result: | Reduced inflammatory cell infiltration and protein accumulation in bronchoalveolar lavage fluid (BALF), reduced MDA levels and increased SOD activity in lung tissue.
Decreased levels of pro-inflammatory cytokine in serum. |
| Animal Model: | Surgical injury in Planaria[2] |
| Dosage: | 1-100 nM |
| Administration: | In cold water for 6 days |
| Result: | Accelerated tissue regeneration in a dose-dependent manner with a TRI50 of 2.5 days. |
References
1. Serhan, C.N.
Novel pro-resolving lipid mediators in inflammation are leads for resolution physiology Nature 510(7503),92-101(2014).
2. Dalli, K.J., Sanger, J.M., Rodriguez, A.R., et al.
Identification and actions of a novel third maresin conjugate in tissue regeneration: MCTR3 PLoS One 11(2),e0149319(2016).
3. Dalli, J., Vlasakov, I., Riley, I.R., et al.
Maresin conjugates in tissue regeneration biosynthesis enzymes in human macrophages Proc. Natl. Acad. Sci. USA 113(43),12232-12237(2016).
