Uses
For the labelling of DNA with radioactive dCTP using random oligonucleotides as primers.
High Prime-labelled probes are used in a variety of hybridization techniques: Southern blots
- Northern blots
- Dot/slot blots
- Screening of gene libraries
- In situ hybridizations
High Prime has been used to label the probes by random hexamer labelling and labelling of probes with
32P by random priming.
General Description
High Prime, a novel labeling mixture, contains optimal concentrations of nucleotides and primers in a highly efficient reaction mix with Klenow enzyme. This convenient "all-in-one" principle of High Prime reduces pipetting steps to a minimum, and increases accuracy and reproducibility of labeling reactions.
