Uses
- Use Anti-HA-Peroxidase for the immunochemical detection of native influenza hemagglutinin protein and recombinant HA-tagged proteins using:
- Dot blot
- Western blot
Use of Anti-HA-Peroxidase eliminates the need for a secondary detection step.
Note: For experiments in which sensitivity is not critical, use Anti-HA-Peroxidase (12CA5). For higher sensitivity detection in western blotting at 10-fold lower concentration, use Anti-HA High Affinity.
General Description
Anti-HA-Peroxidase is a monoclonal antibody to the HA peptide, conjugated to peroxidase. Anti-HA-Peroxidase recognizes the HA nonapeptide sequence (YPYDVPDYA), derived from the human influenza virus hemagglutinin protein (amino acids 98-106). The antibody recognizes its antigenic determinant even when the HA peptide epitope is introduced into unrelated recombinant proteins by a technique known as "epitope tagging".
