Description
BTC is a low affinity calcium indicator (K
d = 7-26 μM) that displays excitation/emission spectra of 401/529 nm, respectively. It exhibits a shift in the excitation maximum from approximately 480 to 401 nm upon calcium binding, enabling determination of ratiometric calcium measurements.
1 BTC is suitable for detecting elevated calcium levels associated with activation of smooth muscle, neurons, and intracellular calcium stores.
1,2
References
1. Sabnis, R.W.
Handbook of biological dyes and stains: Synthesis and industrial applications ,(2010).
2. Hyrc, K.L., Bownik, J.M., and Goldberg, M.P.
Neuronal free calcium measurement using BTC/AM, a low affinity calcium indicator Cell Calcium 24(3),165-175(1998).
